Plant Hsp90 Proteins Interact with B-Cells and Stimulate Their Proliferation
Figure 3
The stimulation ability of rpHsp90 in spleen cells from BALB/c mice.
(A) Proliferative responses of splenocytes to various stimuli. The splenocytes were incubated for 72 h at 37°C in 5% CO2. (B) Kinetics of splenocyte proliferations. The concentrations of the different stimuli were: ConA 5 µg/ml, LPS 10 µg/ml, rLiHsp83 25 µg/ml and rTgHsp28 50 µg/ml. Polymyxin B (PX) at 2 µg/ml was added to the wells containing the recombinant Hsps proteins and LPS. Values represent the mean counts per minute and standard deviations of triplicate samples and are representative of three experiments. ConA: concanavalin A; LPS: lipopolysaccharides; rAtHsp81.2: recombinant heat shock protein 81.2 from Arabidopsis thaliana; rNbHsp90.3: recombinant heat shock protein 90.2 from Nicotiana benthamiana; rLiHsp83: recombinant heat shock protein 83 from Leishmania infantum; rTgHsp28: recombinant small heat shock protein 28 from Toxoplasma gondii. The asterisks indicate the statistically significant differences between the different stimuli and the negative controls (medium, LPS + PX and rTgHsp28). Statistical analysis was performed by one-way analysis of variance (ANOVA) using the Bonferroni's Multiple Comparison Test. *p<0.05, **p<0.01, ***p<0.001.