Identification of Replication Competent Murine Gammaretroviruses in Commonly Used Prostate Cancer Cell Lines
Figure 1
LAPC4, VCaP and EKVX cancer cell lines are infected with a murine leukemia virus which is not XMRV.
(A) Examples of MLV-negative (DU145) and positive (CWR22Rv1, LAPC4, VCaP and EKVX) cancer cell lines stained with MLV30 and MLV70 antisera. (B) Positive staining in LAPC4, VCaP and EKVX does not represent XMRV. Lanes 1–7, PCR with MLV-specific primers (1 = CWR22Rv1, 2 = LAPC4, 3 = VCaP, 4 = DU145, 5 = LNCaP, 6 = PC3, 7 = EKVX), Lanes 8–11, PCR with XMRV-specific primers (8 = CWR22Rv1, 9 = LAPC4, 10 = VCaP, 11 = EKVX). Difference in product size between CWR22Rv1 (lane 1) and LAPC4, VCaP or EKVX (lanes 2,3,7) is due to the XMRV-specific 24-nt deletion [13]. L = molecular weight ladder.