Conservation and Diversity of Seed Associated Endophytes in Zea across Boundaries of Evolution, Ethnography and Ecology
Figure 6
Summary of forward labelled 16S rDNA TRFLP fragments in seeds and stems displayed as presence or absence data.
Fragment sizes are listed on the left side in base pairs, and fragments are noted as being present if amplified in at least 1 of the 3 PCR trials but not the water control. Potential fragment identities were determined by sequencing of isolates or clones, or by submitting raw TRFLP data to APLAUS+. Microbial presence is indicated by coloured shading depending on which plant samples it was observed in, with grey being Generation 1 seed, horizontal black bars being Generation 2 seed, vertical green bars being stem tissue, black being Generation 1 and 2 seed, green being Generation 1 seed and stems, blue being Generation 2 seed and stems, and red being Generation 1 and 2 seed plus stems. Fragments smaller than 25 bp and those representing mitochondrial 18S (536–538 bp) were excluded from the display.