Defective Interfering Viral Particles in Acute Dengue Infections
Figure 2
Effect of the concentration of DENV 2 RNA template and of the primers used for reverse transcription in 3′RACE on the generation of cDNA.
(A) Analysis of cDNA produced following 3′ RACE employing ten-fold dilutions of RNA transcribed from a DENV 2 infectious clone as template and a variety of primer combinations [P1, D2-5′UTR-F and D2-3′UTR; P2, D2-9678-F and adaptor 2; P3, D2-5′UTR-F and adaptor 2; P4, D2-8375-F and D2-8885-R]. (B) cDNA generated following 3′ RACE RT-PCR with RNA from a DENV 2 isolate as template and the RT step performed with or without an oligonucleotide primer and a variety of primer combinations in PCR. [P1, D2-5′UTR-F and adaptor 2; P2, D2-9678-F and adaptor 2; P3, D2-5′UTR-F and D2-3′UTR-R; P4, D2-8375-F and D2-8885-R].