Melanoma Spheroids Grown Under Neural Crest Cell Conditions Are Highly Plastic Migratory/Invasive Tumor Cells Endowed with Immunomodulator Function
Figure 11
SLM8 spheroid cells inhibit PHA-induced T cell activation and proliferation.
PBMCs from MHC-matched healthy donors (n = 3) stimulated with PHA in the presence or absence of SLM8 spheroid (MS) or their adherent (MA) counterparts. A. Inhibition of T cell proliferation as determined by FACS analysis of T-cell CFSE labeling as described under material and methods. Percentages of dividing T cells and the mean number of divisions of the responding cells, proliferation index (PI) are indicated. B. Analysis of T cell death by 7-AAD staining (left panel) and expression of CD25 as a marker of T cells activation (right panel) by flow cytometry. Experiments were performed in duplicate and results are presented as mean values ± SD from 3 independent experiments (*p<0.05; **p<0.001).