Serpin Induced Antiviral Activity of Prostaglandin Synthetase-2 against HIV-1 Replication
Figure 1
Effect of acute HIV infection on PBMC gene expression.
Gene expression profiling of PBMC acutely infected with HIV-1. For signal transduction gene analysis, 105 PBMC were infected with a 0.01 MOI of the primary isolate HIV-1 (HIV 89.6), for 2 h at 37°C. Total RNA was purified two days after infection. A RT-PCR expression array was performed and gene expression of 84 genes from 20 different signal transduction pathways was analyzed. Genes with significant changes in gene expression (p<0.05, n = 3) compared to uninfected vehicle treated controls are shown. Significance was calculated using the ΔΔCt method for three independent experiments.