Mitotic Defects Lead to Pervasive Aneuploidy and Accompany Loss of RB1 Activity in Mouse LmnaDhe Dermal Fibroblasts
Figure 7
LmnaDhe/+ fibroblasts have foci of γH2AX and increased levels of TRP53 indicating DNA damage.
(A–F) Immunodetection of Lamin B (green; A,B, E,F) and γH2AX (red; C–F) in Lmna+/+(A,C,E) and LmnaDhe/+ (B,D,F) fibroblasts. Mutant fibroblast nuclei have several foci of γH2AX throughout both the blebbed and non-blebbed areas of the nucleus (white arrowheads in D and F), in contrast to Lmna+/+nuclei. Single confocal optical sections though the middle of the nucleus are shown. Nuclei are counterstained with DAPI. (G) Western blots of total TRP53 in mutant and Lmna+/+fibroblasts show higher levels of TRP53 in mutant cells than in Lmna+/+ cells. α-Tubulin was used as loading control.