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Deregulation of MYCN, LIN28B and LET7 in a Molecular Subtype of Aggressive High-Grade Serous Ovarian Cancers

Figure 3

Amplification of MYCN and over-expression of MYCN and LIN28B in C5 tumours.

(A) Boxplots depict differential expression of LIN28B and MYCN in different molecular subtypes of serous ovarian cancers AOCS dataset. (B) DNA copy number levels and expression levels of MYCN are highly correlated. C5 samples (coded in red) are predominately distributed in the top right corner of the plot. Samples with segmented copy number log ratio greater than 0.3 were considered to have a gain of MYCN and samples with segmented log ratio less than −0.3 were considered to have a loss. Fisher's exact test was used to compute the statistical significance of the association. (C) Association between MYCN targets and C5 gene sets. MYCN targets were extrapolated from the intersection of two gene lists, (1) genes bound by N-myc in mouse embryonic cell lines and (2) genes with 2-fold increase in expression following transfection of N-myc in mouse embryonic cells (see Supplementary Methods S1). Using data from AOCS or TCGA gene expression sets genes were classified as high or low in C5 tumours versus all other tumours (C1–C4) at p<0.001 by two-sided t-test. The significance of overlap between C5 gene sets and MYCN gene sets was determined by a one-sided Fisher's exact test. Bar plot depicting the association is shown.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0018064.g003