Apelin Attenuates the Osteoblastic Differentiation of Vascular Smooth Muscle Cells
Figure 4
Effects of apelin on MAKP and PI3-K/Akt activation in CVSMCs.
Cell lysates were subjected to Western blotting and incubated with antibodies against p-ERK, ERK, p-P38, P38, p-JNK, JNK, p-Akt, and Akt. The representative results are shown. (A) Cells exposed to 1 nM apelin for 5–60 min, or 50 µM H2O2 for 15 min as a positive control for P38 and JNK activation. (B) Cells incubated with PD98059 (10 µM) or LY294002 (10 µM) for 2 h prior to treatment with 1 nM apelin for 15 min. (C) Cells treated with scrambled APJ siRNA or APJ siRNA in the presence of 1 nM apelin.