Gecko CD59 Is Implicated in Proximodistal Identity during Tail Regeneration
Figure 5
Real-time PCR analysis of CD59 expression in the regenerating spinal cord.
(A) Quantitative results for RT-PCR amplification of CD59 for the spinal cord from L13 to the 6th caudal vertebra for the controls (Nor) and following tail amputation at 1 day, 3 days, 1 week and 2 weeks. Quantities were normalized to endogenous EF-1α expression. Error bars represent the standard deviation (P<0.01). (B) Localization of CD59 mRNA in the spinal cord by in situ hybridization using CD59 antisense RNA probes. L, M, O and P indicate sections of spinal cord segments at 1 day, 3 days, 1 week and 2 weeks post amputation. J indicates control section with sense probe. Scale bars: J-P, 50 µm.