The Base Excision Repair Pathway Is Required for Efficient Lentivirus Integration
Figure 1
Viability of BER glycosylase deletion cell lines treated with DNA damaging agent H2O2.
BER glycosylase deletion cells and matched wild type littermate cells were treated with increasing concentrations of H2O2. Cells were stained with trypan blue and viable cells counted. The percentage of viable cells remaining is shown. (A) Matched wild type, Myh null and Ogg1 null MEFs treated with H2O2. (B) PCR analysis of Myh and Ogg1 genotypes. Primer sets detected wild type Myh (Myh+/+), the Myh deletion construct (Myh−/−), wild type Ogg1 (Ogg1+/+) and the Ogg1 deletion construct (Ogg1−/−). PCR targets included water (No target) and genomic DNA from wild type (WT), Myh−/−, and Ogg1−/− cell lines. (C) Matched wild type and Neil1 null MEFs treated with H2O2. (D) PCR analysis of Neil1 genotypes. Primer sets detected wild type Neil1 (Neil1+/+) and the Neil1 deletion construct (Neil1−/−). PCR targets included water (No target) and genomic DNA from wild type (Neil1+/+) and Neil1−/− cell lines.