A Defined, Feeder-Free, Serum-Free System to Generate In Vitro Hematopoietic Progenitors and Differentiated Blood Cells from hESCs and hiPSCs
Figure 3
Characterization of the developmental potential of hESC-derived hematopoietic progenitors.
FACS analysis and corresponding cytospins of mature cells generated in culture from hESC-derived progenitors induced with defined factors. Images were taken at 40× magnification. Cytospins of erythroid cells, dendritic cells, macrophages and neutrophils were stained with Wright. Cytospins of megakaryocytes were stained for GPIIb/IIIa (CD41a). Cytospins of mast cells were stained for tryptase. The yield hESC:mature cell indicates the number of each mature cell type generated per each hESC induced to differentiate.