Interactions between the Nse3 and Nse4 Components of the SMC5-6 Complex Identify Evolutionarily Conserved Interactions between MAGE and EID Families
Figure 7
Binding of different MAGE proteins to NSE4a and NSE4b/EID3 proteins.
(A) Cell-free extracts of mouse testes were immunoprecipitated with either IgG or anti-NSE4b followed by Western blotting with the indicated antibodies. (B) S-tagged MAGEA1 or MAGEG1 were co-expressed with FLAG-tagged NSE4b in HEK293 cells. In lanes 1–6 and 13–18, extracts were precipitated with S-protein, whereas in lanes 7–12 they were immunoprecipitated with anti-FLAG antibody. (C–H) S-tagged Class I MAGE protein A1 (C), and class II MAGE proteins D4b (D), F1 (E), G1 (F), necdin (G) or vector alone (H) were co-transfected with FLAG-tagged NSE4a (N4a) (lanes 1–3) or NSE4b/EID3 (N4b) (lanes 4–6). Extracts were immunoprecipitated with S-protein and Western blotted with S-HRP and anti-FLAG antibody.