The RHO-1 RhoGTPase Modulates Fertility and Multiple Behaviors in Adult C. elegans
Figure 3
(A) The number of unlaid eggs remaining in animals was determined as a steady-state measure of egg laying. Expression of hsRHO-1(G14V) (nzIs1) or nRHO-1(G14V) (nzIs29) led to a significant decrease in the number of eggs remaining in animals when compared to wild type controls. Inhibition of endogenous RHO-1 using hsC3 transferase (nzEx4) did not have any significant effect on the number of eggs remaining in animals. (B) The stage of eggs laid was determined by visual inspection. Experiments were repeated four times and the data presented is the sum of these experiments. Animals expressing activated hsRHO-1(G14V) (nzIs1) or nRHO-1(G14V) (nzIs29) laid some early stage eggs containing fewer than eight cells and hsRHO-1(G14V) (nzIs1) the total number of eggs laid was decreased. This decrease was also observed in animals expressing hsC3 transferase (nzEx4).