Immunological and Metabolomic Impacts of Administration of Cry1Ab Protein and MON 810 Maize in Mouse
Figure 4
A–C: Electrophoretic pattern and IgE-binding analysis of protein extracts from MON810 and non-GM maize:
Protein extracts from MON 810 and its conventional counterpart were separated on 12% acrylamide gel and proteins were stained (A). After transfer to PVDF membrane, IgE binding proteins (i.e. allergens) were revealed by western blotting with the sera of 2 maize-allergic patients, i.e. #20770-MH (B) and #19392-CS (C). Specific IgE concentrations (D) in the same sera were determined on GM (open bar) and non-GM (black bar) protein extract coated plates. Bars represent mean+/−SEM obtained for 3 different dilutions of each serum, each assayed in duplicate.