Overexpression of Full-Length ETV1 Transcripts in Clinical Prostate Cancer Due to Gene Translocation
Figure 2
Characterization of the EST14 to ETV1 gene fusion in prostate cancer G270.
(A) Schematic representation of ETV1-EST14 fusion transcripts in prostate tumors G270 and G342 (sample G342 is from ref. 10). Arrows indicate positions of primers used in the RT-PCR experiment. Primer sequences are shown in Supplementary Table S1. (B) Sequence of the fusion point of EST14 and ETV1 in sample G270. The position of the fusion point in tumor G270 was mapped by long-range PCR on genomic DNA with a forward primer in the EST14 intron and reverse primer upstream of ETV1 exon 1. At the fusion point two T residues were lost. The breakpoint in EST14 in G270 and G342 are indicated by arrows.