Crystal Structure of Spy0129, a Streptococcus pyogenes Class B Sortase Involved in Pilus Assembly
Figure 6
In vitro polymerisation assay.
A) Immunoblot with polyclonal antibodies against Spy0128, after mixing Spy0128 and Spy0129 and incubating at 37°C for 20 h, showing the lack of a ladder of high molecular weight bands characteristic of polymer formation. B) Immunoblot of S. pyogenes M1 SF370 pili, composed mainly of polymerised Spy0128, probed with Spy0128 antibodies.