Plasma Membrane Factor XIIIA Transglutaminase Activity Regulates Osteoblast Matrix Secretion and Deposition by Affecting Microtubule Dynamics
Figure 7
FXIIIA inhibition by NC9 reduces the levels of Synaptotagmin VII, secretory vesicle marker, on the plasma membrane.
(A) Immunofluorescence staining of the secretory vesicle marker synaptotagmin VII (Syt VII) and it's colocalization with plasma membrane tubulin in control (DM) and NC9-treated cells. NC9 treatment markedly lowers Syt VII levels and colocalization with tubulin on the plasma membrane. (B) Syt VII levels are significantly lower on the cell surface of NC9-treated cells and decreased down to 29% of controls. Levels were quantified by IMAGE-J software (version 1.37a, National Institutes of Health, USA). Error bars represent s.e.m. of intensity otained from three separate areas in the images.