Plasma Membrane Factor XIIIA Transglutaminase Activity Regulates Osteoblast Matrix Secretion and Deposition by Affecting Microtubule Dynamics
Figure 5
Immunofluorescence colocalization of NC9 with FXIIIA and TG2.
(A) Intracellular immunofluoresence colocalization (yellow) of NC9 with FXIIIA and TG2 (both green) shows complete colocalization of the inhibitor with FXIIIA, but no colocalization with TG2. The dansyl group of NC9 was stained with an anti-dansyl antibody followed by anti-rabbit Alexafluor 568 conjugate (orange). (B) Extracellular colocalization (nonpermeabilized cells) of NC9 with FXIIIA and TG2 shows complete colocalization (yellow) of the inhibitor with FXIIIA and limited colocalization with TG2 in a patch-type staining pattern. (C) Colocalization of FXIIIA with TG2 (yellow) showed that these two enzymes are found together on the cell surface – FXIIIA in larger patches and TG2 in small vesicles.