C. elegans Mutant Identification with a One-Step Whole-Genome-Sequencing and SNP Mapping Strategy
Figure 1
Principle of the WGS-SNP strategy.
The red diamond indicates the mutation of interest. The sequence ‘pileup’, generated by processing the WGS sequencing data, is a representation of the number of reads that are mapped to a specific position in the genome. The relative number of N2 vs. Hawaiian nucleotides is a reflection of the relative distribution of recombinants in the pool.