The Prevalence and Regulation of Antisense Transcripts in Schizosaccharomyces pombe
Figure 3
Differentially expressed sense and antisense transcripts.
(A) Strand-specific RT-PCR results of four loci selected for validation. PCR products specific to antisense (AS) or sense (S) transcripts are shown. Positive control (T; RT with two gene-specific primers) and negative control (-; no primer at RT step) are also included. PP: S≥ AS in both normal (NM) and heat shock (HS) conditions; PN: S≥ AS in NM and S< AS in HS; NP: S< AS in NM and S≥ AS in HS; NN: S< AS in both NM and HS. (B) Correlation between DeLi-seq and strand-specific qPCR. The log2(S/AS) values obtained by DeLi-seq (X axis) and the ΔCt values between sense and antisense transcripts determined by quantitative strand-specific RT-PCR (Y axis) were used to compute the correlation coefficient (R). (C) Sense and antisense transcripts are either coordinated (correlated or anti-correlated) or independently regulated. The number of genes in each category is shown.