CXCR6, a Newly Defined Biomarker of Tissue-Specific Stem Cell Asymmetric Self-Renewal, Identifies More Aggressive Human Melanoma Cancer Stem Cells
Figure 2
Flow cytometry detection and sorting of ABCG2+ subpopulations from cultures of human melanoma cell lines.
Primary melanoma IGR39 cells and metastatic melanoma IGR37 cells were incubated with the indicated fluorescent FITC-conjugated antibodies and analyzed by flow cytometry as described in Materials and Methods. Y-axes, side scatter; x-axes, relative fluorescence intensity. Left panels, analyses with isotype (IgG) control antibodies; middle panels, analyses with anti-ABCG2 FITC-conjugated antibodies. After FACS isolation based on the indicated gate (blue outline), ABCG2+ sorted cells were reanalyzed to confirm enrichment (right panels). Numbers, percent of total evaluated cells.