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Perifosine and CCI 779 Co-Operate to Induce Cell Death and Decrease Proliferation in PTEN-Intact and PTEN-Deficient PDGF-Driven Murine Glioblastoma

Figure 2

Combing perifosine and CCI-779 effectively inhibits both the Akt and mTOR pathways in primary glioma cultures treated in vitro.

(A) A representative immunoblot of PTEN +/+ primary GBM cell cultures after 4 hours with no treatment (NT), vehicle (V), 30 µM perifosine (P), 1 nM CCI-779 (C) or combination of 30 µM perifosine and 1 nM CCI-779 (P+C). (B) Quantification of the changes in pAkt and pS6RP based on three independent primary PTEN +/+ GBM cell cultures, with 20 µg protein per well. (C) A representative immunblot of PTEN -/- primary GBM cell cultures after 4 hours with no treatment (NT), vehicle (V), 30 µM perifosine (P), 1 nM CCI-779 (C) or combination of 30 µM perifosine and 1 nM CCI-779 (P+C). (D) Quantification of the change in pAKT and pS6RP based on three independent primary PTEN -/- GBM cultures, with 20 µg protein per well.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0014545.g002