Early Acquisition of Neural Crest Competence During hESCs Neuralization
Figure 6
Spontaneous differentiation of emNCSCs.
(A) Peripheral neuron-like cells are co-stained with TH (green) and Peripherin (red). Individual channels are shown in the inserts.(B) Smooth muscle cells reactive to smooth muscle actin. (C) The addition of 1% horse serum to the NS medium yielded GFAP-positive cells co-expressing p75NTR and proteolipid protein (PLP), consistent with Schwann cell identity. Inset, PCR detection of GFAP transcript. CTRL = NS medium. Scale bar = 25 ìm. (D) Brn3a (red) and Peripherin (green) staining indicates differentiation into peripheral sensory-like neurons. (E) Bright field image of black inclusion bodies in a characteristic melanosome, inset box shows Melan A (green) reactivity. Nuclei were visualized by DAPI. Scale bars represent 40 µm in A, C and D, 60 µm in D, and 100 µm in E and its inset.