Selective Chemokine Receptor Usage by Central Nervous System Myeloid Cells in CCR2-Red Fluorescent Protein Knock-In Mice
Figure 4
CCR2-deficient mice exhibit delayed onset EAE and decreased monocyte recruitment to the CNS.
(A) Ccr2RFP/+Cx3cr1GFP/+ and Ccr2RFP/RFPCx3cr1GFP/+ mice were immunized with MOG peptide 33–55 and scored daily for neurological signs. (B) EAE symptom onset and peak disease. (C) Total number of brain mononuclear cells and CD45hi infiltrating cells at peak disease. (D) CD45hi infiltrating cells were analyzed to quantify total neutrophils (SSChi, CD115−, Ly6Clo), monocytes (CD115+), and monocyte subsets (CD115+/Ly6Chi/lo), (E) CX3CR1 GFP and CCR2-RFP fluorescence intensities in monocyte subsets and microglia. Data points represent individual mice. Values are mean ± SEM. Results are representative of two similar experiments.