cis-Urocanic Acid Attenuates Acute Dextran Sodium Sulphate-Induced Intestinal Inflammation
Figure 4
Effect of cis-UCA on lipopolysaccharide (LPS)-induced cytokine secretion by bone-marrow-derived antigen presenting cells (BM-APC).
BM-APCs derived from long-bones of 129 Sv/EV mice (1×106 cells/ml) were pre-treated for 2 hrs with cis-UCA (5 µg, 50 µg, or 100 µg) and then stimulated with LPS (1 µg/ml). Supernatants were collected after 24 hrs. Unstimulated BM-APCs were CD11b+, CD11c+ and F4/80− (A). LPS induced TNFα secretion from BM-APCs and this was not altered by the presence of cis-UCA (B). LPS also induced CXCL1 secretion by BM-APCs and this secretion was attenuated by cis-UCA (C). n = 4–13 for all measurements. *: p<0.01 compared with LPS.