The Nutritional Induction of COUP-TFII Gene Expression in Ventromedial Hypothalamic Neurons Is Mediated by the Melanocortin Pathway
Figure 6
COUP-TFII gene expression in response to the MC4-R activation in GT1-7 cell line.
GT1-7 cells were cultured for 18 hours in the presence of either (A) insulin (100 nM) or increasing amount of (B) MTII or (C) DiBcAMP. Results are expressed as a fold increase over the value obtained without MTII (A) or without DiBcAMP (B). (D) Transient transfection of a minimal COUP-TFII promoter in response to DiBcAMP. Data are expressed as the mean ± SEM. (n = 4). *P≤0.05 **P≤0.01 when compared to cells cultured in the absence of MTII or DiBcAMP.