Immunomodulation Targeting Abnormal Protein Conformation Reduces Pathology in a Mouse Model of Alzheimer's Disease
Figure 7
Immunostaining of AD tissue by plasma from immunized mice.
Panel A shows immunolabeling using plasma (T6) from pABri vaccinated Tg mice on normal, aged brain sections. No specific immunolabeling is seen. In AD temporal cortex sections extensive cytoplasmic neuronal immunoreactivity is evident, shown in B. Absorption of the immune plasma with aggregated Aβ1-42 resulted in loss of neuronal immunolabeling, shown in C. D shows a higher magnification of the lack of specific labeling with plasma from a pABri vaccinated Tg mouse in normal brain tissue. In E a higher magnification of the neuronal cytoplasmic and dendritic immunolabeling is shown (see arrows). F depicts doubling labeling where the neuronal staining with the immune plasma is seen in black and neurofibrillary tangle immunolabeling with PHF-1 is seen in red (see arrows). Hence some neurofibrillary tangles immunolabeled with both PHF-1 and the plasma from pABri vaccinated mice. In G immunostaining with plasma which had been absorbed with aggregated Aβ1-42 on an AD temporal cortex section is shown. H shows immunolabeling in a sequential section to G using plasma which had been eluted from the aggregated Aβ1-42 pellet. I shows immunolabeling with PHF-1 in a sequential section. The arrows show labeling of dystrophic neurites with the eluted immune mouse plasma in H and with PHF-1. J, K and L depict a similar series of pictures using a different plasma sample from a Tg mouse immunized with pABri, showing a lack of specific labeling in J, immunolabeling of dystrophic neurites with the eluted plasma sample in K and the matching PHF1 labeling in L. Rare amyloid β plaques also showed faint immunolabeling with plasma from pABri immunized mice, as shown in M (see arrows). N shows a double immunolabeling where the plasma from a pABri immunized mouse is labeling neuronal perikarya and dendrites (see black arrows) and Aβ immunoreactivity is seen in red (see red arrows). There is no co-localization of the plasma immunolabeling with the anti-Aβ 4G8/6E10 immunolabeling. O depicts a lack of specific immunolabeling at a lower magnificent of a similar temporal cortex AD section to M and N where the pre-immune (T0) plasma was used from the same mouse.