dATP/ATP, a Multifunctional Nucleotide, Stimulates Bacterial Cell Lysis, Extracellular DNA Release and Biofilm Development
Figure 1
Confocal laser scanning micrographs of biofilms developed by E. coli K-12 in 10% LB broth with different reagents.
A, control, without any additional reagents; B, with 400 µM of dATP;, C, with 40 U/ml of DNase I; and D, with 200 mU/ml of Apyrase. Biofilms were examined after 16 hrs incubation after inoculation at 30°C in a 96-well microtiter plate. Bacterial cells were stained with Bacterial LIVE/DEAD staining dyes and viable cells shown green and dead or membrane damaged cells shown red in the images.