The C-H Peripheral Stalk Base: A Novel Component in V1-ATPase Assembly
Figure 4
Stabilization of the catalytic interfaces.
(A) A top view of the CE3G3H complex fit with a 50% transparent representation of the A3B3DF sub-assembly (PDB 3A5D). X-ray coordinates of the EG heterodimer from T. thermophilus (PDB 3K5B) were fit into the density map to discern the extent of the three peripheral stalk boundaries. Based on their orientation, the peripheral stalks (green and lime green) are seen interacting with the non-catalytic interfaces between the A (orange) and B (yellow) subunits [13], [17], [46]. This arrangement allows for the nucleotide-binding interfaces (NBI) to be openly accessible for binding to ATP and ADP while also facilitating enough space for rotation of the central stalk (subunit D, cyan).