Fluorescent Labeling of Newborn Dentate Granule Cells in GAD67-GFP Transgenic Mice: A Genetic Tool for the Study of Adult Neurogenesis
Figure 4
GFP+ granule cells express newborn neuron markers.
a-b″, Confocal images show GFP+ cells do not colocalize with glial cell and neuronal stem cell marker GFAP (a-a″) or proliferating cell marker Ki67 (b-b″). c-d″, GFP+ cells precisely colocalize with newborn neuron makers PSA-NCAM (c-c″) and DCX (d-d″). e-e″, Many GFP+ cells are weakly positive for neuronal marker NeuN (closed arrowheads) while others are either negative (open arrowhead) or strongly positive (arrow). f, Quantification of colocalization of GFP+ cells with various cellular markers (mean±SEM). Images are single optical sections. Scale bar, 20 µm.