Paeonol Oxime Inhibits bFGF-Induced Angiogenesis and Reduces VEGF Levels in Fibrosarcoma Cells
Figure 4
PO disrupts bFGF-induced angiogenesis in vivo.
PO and bFGF were loaded on CAMs of 10-day-old fertilized chicken eggs. After 72 h incubation, a fat emulsion was injected into the CAMs for better visualization of the blood vessels. Thermanox disc and surrounding CAMs were photographed. (A) Untreated control, (B) bFGF-treated control, (C) 0.25 µg of PO with bFGF, and (D) 0.5 µg of PO with bFGF. (E) The number of newly formed blood vessels per field was counted. All data are presented as mean ± S.D., n = 5. Statistically significant differences between control and sample groups were calculated by Student's t-test. ##, p<0.05 versus untreated control.*, p<0.05 and ***, p<0.001 versus bFGF control.