The ROCK Inhibitor Y-27632 Improves Recovery of Human Embryonic Stem Cells after Fluorescence-Activated Cell Sorting with Multiple Cell Surface Markers
Figure 3
Recovery of hESCs grown feeder-free after sorting and passaging without and with Y-27632.
Percent recovery of H9 hESCs at day 1 after A) sorting or B) passaging with Accutase. Percent recovery equals the number of cells recovered at day 1 after passage or sorting divided by the number of cells plated. A total of two (Trial 3) to three wells (Trials 1, 2 and 4) were counted for each trial group with error bars representing standard deviation. C) Fold increase in the recovery of cells at day 1 upon addition of Y-27632 after sorting. The number of cells recovered without Y-27632 was set at 1. D) Percent recovery at day 1 post-sort after pre-treatment of cells with Y-27632 and Y-27632 post-sort titration. A total of three wells were counted for each trial group with error bars representing standard deviation.