Protection of Macaques with Diverse MHC Genotypes against a Heterologous SIV by Vaccination with a Deglycosylated Live-Attenuated SIV
Figure 7
Recombination between vaccine and challenge virus.
Nucleotide sequences of SIV fragments amplified by sets of nested PCR using primers based on SIVsmE543-3 or SIVmac239 to cover the entire SIV genome except the 5′ and 3′ terminal sequences (∼100 bp). Representative sequences from integrated results of multiple sequences of PCR fragments are shown. The sequences detected in Mm0304 at 25 weeks pc were the vaccine virus sequences (top bar indicated by open box) and the sequences detected in Mm0513 at 55 weeks pc were the challenge virus sequences (bottom bar indicated by grey box) except in the case of the latter which contained a 9 bp deletion shown by triangle. Other represented sequences were recombinant viruses between vaccine (open boxes) and SIVsmE543-3 (gray boxes). Lines with arrowheads indicate the sequences that were targeted for nested PCR to quantify the recombinant viruses and SIVsmE543-3 in the non-controllers.