miR-148 Regulates Mitf in Melanoma Cells
Figure 3
Mutating miRNA binding sites affects the Mitf mRNA.
A. Effects of miR-148 on the mouseMitf-3′UTR-luciferase reporter when the potential binding sites are mutated. Results in all panels are presented as mean values ±SD. Difference between the appropriate vector (labeled on the X-axis) with and without co-transfected miRNA was considered statistically significant when p<0.05, shown with an asterisk (T-test, 95% confidence level). P-values are presented in Table S1. B. Effects of miR-137 on the mouseMitf-3′UTR-luciferase reporter when the potential binding sites are mutated. C. Effects of miR-124 on the mouseMitf-3′UTR-luciferase reporter when the potential binding sites are mutated.