Mitochondrial DNA Mutations Induce Mitochondrial Dysfunction, Apoptosis and Sarcopenia in Skeletal Muscle of Mitochondrial DNA Mutator Mice
Figure 5
No differences between WT and D257A mice in ETC complexes I, II, IV and F1-ATPase specific activity.
(A) The activity of ETC complexes I, II, IV and the F1 domain of the ATPase, in skeletal muscle of 11-mo old WT and D257A mice (n = 7 per group), was determined using Blue Native PAGE, followed by enzymatic colorimetric reactions performed on the gels. Representative blots are depicted. (B) Statistical analysis of ETC complexes specific activity. Arbitrary units represent activity densitometry values normalized to respective content densitometry values for each sample. No statistically significant differences were observed. Mean values ±SEM are shown.