Fine Mapping the Spatial Distribution and Concentration of Unlabeled Drugs within Tissue Micro-Compartments Using Imaging Mass Spectrometry
Figure 1
The distribution of tiotropium on two central lung tissue sections from a rat after inhalation dosing.
The arrows indicate the route of drug delivery. (A) Photographic image of a rat lung tissue section analyzed by MALDI IMS with (B) the obtained distribution of tiotropium represented by the parent ion m/z 392.1. (C) A typical MS spectra obtained by MALDI MS analysis on rat lung tissue. (D) Photographic image of a rat lung tissue section analyzed by MALDI IMS/MS with (E) the obtained distribution of tiotropium represented by the daughter ion m/z 152.1. (F) MS/MS spectra of tiotropium. The distribution of tiotropium obtained in MS mode (B) correlate very well with the distribution pattern obtained by rastering over the tissue in MS/MS mode (E). The MALDI matrix CHCA was applied by an automatic sprayer device.