Human Adipose Derived Stromal Cells Heal Critical Size Mouse Calvarial Defects
Figure 6
Bone turnover within a calvarial defect.
(A, far left column) FISH staining for human sex chromosomes in hASC engrafted defects from 1–8 weeks postoperatively. Human X chromosome appears green, while DAPI nuclear counterstain appears blue. 100× magnification. (A, mid left column) TUNEL staining in hASC engrafted defects from 1–8 weeks postoperatively. TUNEL positive cells appear green, while DAPI nuclear counterstain appears blue. (A, far right column) TRAP staining in hASC engrafted defects from 1–8 weeks postoperatively. TRAP positive cells appear red/purple. (B) Luciferase activity in defects engrafted with Luc+ mouse ASCs. Images from a representative mouse (left), and quantification (right). Red represents the highest level of activity, followed by yellow, blue, dark blue, and purple. N = 3 animals. *P<0.01.