Biochemical Characterization of Enzyme Fidelity of Influenza A Virus RNA Polymerase Complex
Figure 5
Effect of H3N2 NP on polymerase activity and the misincorporation efficiency of the IAV Pol complex.
(A) IAV polymerization in the presence and absence of varying concentrations of H3N2 NP with the 50-mer template used in Figure 1B. Molar ratios between the RNA template and NP molecule were altered between 0 and 2. The RNA template was preincubated with NP before adding the polymerase complex. LC: loading control; (B) Fully extended products for the IAV Pol activity with different molar ratios of RNA/NP were compared after being normalized with the value obtained with no NP (100%). (C) The misincorporation efficiency of IAV Pol was calculated as described in Figure 2F, and compared using the value obtained with no NP (100%). C: no polymerase control.