Identification of Genes Involved in Polysaccharide-Independent Staphylococcus aureus Biofilm Formation
Figure 1
Extracellular protease activity of biofilm mutants.
Protease activity in cell-free supernatants from cultures grown in TSB for 12 hours was measured with Azocoll reagent as described in Materials and Methods. Supernatant protease activity of the wild-type strain (SH1000) was set to 1 as a reference. The graph shows the mean of three samples; error bars show standard deviation. Blue bars indicate mutants that show consistently higher levels of protease activity.