Two-Photon Microscopy for Non-Invasive, Quantitative Monitoring of Stem Cell Differentiation
Figure 2
TPEF analysis of hMSCs following KCN or FCCP treatment.
The inverse fluctuations of NAD(P)H and FP fluorescence in response to KCN or FCCP treatment were detectable via either TPEF emission spectra excited at 755 nm, or TPEF images excited at 755 nm and 860 nm. For each method, means are displayed relative to values before treatment with standard error bars. Spectral data from multiple microscopic fields (N = 9) were used for the analysis of the TPEF emission spectra while the results from TPEF images were calculated on a cell by cell basis from multiple image sets (N = 42).