Two-Photon Microscopy for Non-Invasive, Quantitative Monitoring of Stem Cell Differentiation
Figure 1
Components of hMSC TPEF emission spectra.
(A) hMSC TPEF emission spectra collected at either 755 nm or 860 nm excitation with corresponding fits by the ALS script. (B) linear unmixing methods reveal the contributing cellular fluorophores to be NAD(P)H, Lipofuscin and FP. When components of intrinsic cellular fluorescence are used to produce the fits displayed in panel A the NAD(P)H component does not contribute to cellular spectra excited at 860 nm.