Immunoproteomics Analysis of the Murine Antibody Response to Vaccination with an Improved Francisella tularensis Live Vaccine Strain (LVS)
Figure 2
Two-dimensional immunoblots of Francisella tularensis LVS protein extracts hybridized with sera from C57BL/6 mice unsuccessfully vaccinated with DVC-LVS Lot16.
(a) Representative silver stained reference 2D-PAGE of LVS total protein lysates separated in pH range 4–7 and b) equivalent immunoblot. (c) LVS membrane enriched fractions, separated in pH range 4–7 and (d) corresponding immunoblot. Immunoreactive areas are labelled on Western blot images and the corresponding immunoreactive proteins are indicated on silver stained gels in (a) and (c). The annotation numbers indicate the protein locus tag and are summarized in Table 1. Two spurious areas on blot (d) were observed and did not correspond to areas of immunoreactivity.