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Sensitive Detection of Gene Expression in Mycobacteria under Replicating and Non-Replicating Conditions Using Optimized Far-Red Reporters

Figure 11

Stability of M. tuberculosis fluorescent strains after long-term excitation.

M. tuberculosis transformants expressing FPs from Psmyc were grown in liquid medium and the OD580 adjusted to 0.01. Fluorescence was quantified after excitation over a period of 20 min. (▴) mCherry, (▪)Turbo-635 and (□) tdTomato.

Figure 11

doi: https://doi.org/10.1371/journal.pone.0009823.g011