Sensitive Detection of Gene Expression in Mycobacteria under Replicating and Non-Replicating Conditions Using Optimized Far-Red Reporters
Figure 5
Far-red fluorescent reporters are functional in M. marinum strain M.
Fluorescent reporters were expressed from Psmyc in M. marinum and assayed in liquid culture grown either in the dark or in the light. Cultures were diluted (from left to right) to an OD580 of 0.25, 0.10, 0.05 and 0.01, representing cell densities of 9.8×105, 4.2×105, 1.9×105 and 6.5×104 CFU/ml respectively. Fluorescence was quantified at the following wavelengths: mCherry 587/610 nm, Turbo-635 588/635 nm. Data are the mean and standard deviation from three independent transformants. A value of 1015 corresponds to saturation of the machine. The reporter and promoter backbone are indicated.