Versatile Virus-Like Particle Carrier for Epitope Based Vaccines
Figure 5
Immunogenicity of D2 peptide displayed on AP205 VLPs.
AP205 VLPs with the D2 peptide fused to the C-terminus (AP418, short linker, and AP420, long linker) and to the N-terminus (AP421, short linker, and AP422, long linker) were injected s.c. at a dose of 25 µg twice, on day 0 and 14. Sera were collected on day 21, and IgG titre measured by ELISA. Pre-immune titre was too low to be determined, and was arbitrarily attributed the value of 1∶100, the lowest dilution of serum used in the assay.