A Monomeric Variant of Insulin Degrading Enzyme (IDE) Loses Its Regulatory Properties
Figure 2
Comparison of the molecular weight forms of wild type IDE and IDEΔC on a Superdex S200 gel filtration column.
Wild type IDE or the IDEΔC variant was chromatographed on a Pharmacia Superdex S200 gel filtration column in 20 mM potassium phosphate buffer, pH 7.4. One ml of protein at 0.5 mg/ml was loaded and the column developed at a flow rate of 0.4 ml/min. Fractions were collected and assayed for IDE activity using Abz-GGFLRKHGQ-EDDnp as the substrate.