Hypoxia Promotes Glycogen Accumulation through Hypoxia Inducible Factor (HIF)-Mediated Induction of Glycogen Synthase 1
Figure 4
Hypoxia increases glycogen synthase activity.
c2c12 myoblasts were incubated at 21% (Nx) or 1% oxygen (Hx) for the indicated periods of time and processed to determine GYS1/pGYS1(phospho-Ser 641) levels (A) and glycogen synthase activity in the absence (B) or presence of 6.6 mM of the allosteric modulator glucose-6-phospate (C). Graphs represent the mean of duplicated measures and error bars the range. (D) The graph represents the ratio of glycogen synthase activity in the absence (I) and presence (T) of glucose-6-phosphate. The experiment was repeated twice with similar results.