In Vitro and Ex Vivo Testing of Tenofovir Shows It Is Effective As an HIV-1 Microbicide
Figure 1
Microbicide pre-clinical testing algorithm.
Tenofovir and vehicle control gels were evaluated through a comprehensive pre-clinical algorithm. The algorithm focuses on testing the formulation's physiochemical properties and the ability to release the drug; in vitro testing that includes safety of vaginal flora, epithelial and immune cells, and efficacy against multiple HIV-1 clades; and ex vivo testing using ectocervical and colorectal explants to evaluate drug absorption and formulation safety and efficacy against HIV-1. The data obtained from this algorithm along with data supplied by the manufacturer aid in the decision to continue testing the product.