A Novel Probiotic Mixture Exerts a Therapeutic Effect on Experimental Autoimmune Encephalomyelitis Mediated by IL-10 Producing Regulatory T Cells
Figure 5
A synergistic combination of probiotics induces Treg cell expansion and systemic IL-10 production.
Healthy C57BL/6 mice were daily fed with L. paracasei DSM 13434, L. plantarum DSM 15312, L. plantarum DSM 15313, a mixture of all three (Lacto-mix) or saline (control) during 14 days (n = 5). MLNs and spleens isolated from these animals were analysed for expression of CD3, CD4, CD25 and Foxp3 by flow cytometry. (A) The proportion of CD4+CD25+Foxp3+ stained cells was examined on the total gated CD3+ lymphocytes from MLNs (top) and spleen cells (bottom). Blood serum from these animals was analysed for different cytokines using ELISA kits. Shown are data from (B) IL-10, (C) TGF-β1 and (D) IL-27 analysis. Data are mean ± SEM and representative of two experiments. * represents a p-value ≤0,05 and ** a p-value ≤0,01.